Protocols
Below you can download protocols that I have used in the laboratory and bioinformatic work.
No promises, but thay worked for me. Good Luck!
DNA & RNA isolation
DNA from Reptile Blood & Tissue....
RNA for Illumina RNA-seq..............
PCR, Sequencing ............................
TA Cloning .....................................
PCR, Sequencing, Cloning
Lab Work Flows
Parentage with microsatellites.........
Sequence Capture to Sequencing......
Galaxy Workflows.
Cleaning Illumina Reads: http://main.g2.bx.psu.edu/u/ts-ecogen/w/groomilluminasolexatrimq20filter20bp
Mapping Illumina reads to candidate genes: http://main.g2.bx.psu.edu/u/ts-ecogen/w/map-illumina-indiv-to-candidate-transcripts-and-count
Bioinformatic Workflows