Protocols

Below you can download protocols that I have used in the laboratory and bioinformatic work.

No promises, but thay worked for me. Good Luck!

DNA & RNA isolation

 

DNA from Reptile Blood & Tissue.... 

 

 

RNA for Illumina RNA-seq..............

 

 

 

 

 

 

 

 

 

PCR, Sequencing ............................

 

 

TA Cloning  .....................................  

 

 

 

 

 

 

 

 

 

PCR, Sequencing, Cloning

 

Lab Work Flows

 

Parentage with microsatellites.........

 

Sequence Capture to Sequencing......

 

 

 

 

 

 

 

 

 

Galaxy Workflows. 

 

Cleaning Illumina Reads: http://main.g2.bx.psu.edu/u/ts-ecogen/w/groomilluminasolexatrimq20filter20bp

 

Mapping Illumina reads to candidate genes: http://main.g2.bx.psu.edu/u/ts-ecogen/w/map-illumina-indiv-to-candidate-transcripts-and-count

 

 

 

 

 

 

 

 

 

Bioinformatic Workflows

 

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